ribaoo Research Daily

RISK / BOUNDARY

Metabolic Mechanisms of INSTI-Associated Weight Gain: A Multi-Omics Study in Male People with HIV.

Integrase Strand Transfer Inhibitors (INSTI) are recommended as first-line antiretroviral therapy (ART) for HIV infection, yet are frequently associated with excessive weight gain. We conducted a multi-omics study on male people with HIV (PWH). A total of 64 male PWH were enrolled and stratified based on their therapeutic history and weight changes over the first year (G1 receiving INSTI with weight gain ≥ 5%; G2 switching to INSTI with weight gain ≥ 5%; and G3 switching to INSTI without weight gain). INSTI-based regimens included bictegravir-, elvitegravir-, and dolutegravir-containing combinations. 26 healthy male controls were also included. Plasma untargeted metabolomics and proteomics were analyzed longitudinally. Machine learning models were employed to integrate multi-omics data and identify predictive biomarkers. Metabolomic analysis revealed that switching to INSTI induced a generalized hypometabolic state, characterized by a universal decline in thyroxine and an increase in N-acetylputrescine across all switching groups, independent of weight outcomes. In contrast, proteomic profiling identified distinct molecular drivers specific to the weight-gain groups. Random forest modeling identified transthyretin (TTR) as the top predictive biomarker among pre-INSTI multi-omics features. Our integrated multi-omics approach reveals that INSTI-based regimens induce universal metabolic changes. The change of thyroxine and the predictability of TTR in multi-omic studies show the critical role of thyroxine metabolism in INSTI-associated weight gain.

Evidence boundary: 有限样本中的关联与预测特征不能证明单一机制。

Source: PMID:42507986

LATEST VISIBLE BATCH

Consuming my body weight in videos: The relationships between watching social media eating videos, body image and eating behaviour among Australian and Chinese individuals.

Observation date: 2026-07-28. The primary-source endpoint did not yield a verifiable new batch in this run; this issue uses the latest visible record from 2026-07-27, not a publication-date claim for the observation date.

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Eating videos have become increasingly popular on social media. Research has shown that eating videos such as mukbang (eating broadcasts where hosts eat a large amount of food) may be linked to negative body image and disordered eating. However, little is known about other types of eating-related content (e.g., eating challenges). The aim of the current study was to: (1) investigate the relationship between watching a range of eating videos (e.g., mukbang, eating challenge), body image, and eating behaviours, and (2) examine the indirect effects of appearance comparison and thin-ideal internalisation. In addition, given the popularity of eating videos in certain Asian cultures, we compared differences between Australian and Chinese individuals. Participants (82.3% women; 257 Australian, 189 Chinese) aged 18-35 years completed an online cross-sectional questionnaire. Results showed that viewing content such as mukbang and eating challenges was positively associated with various body image and eating concerns (e.g., thin-ideal internalisation, food craving, bulimia). Both appearance comparison and internalisation of appearance ideals accounted for significant indirect effects in the relationships between watching certain eating videos, disordered eating and food craving. There were differences between Australian and Chinese individuals; for example, Chinese participants reported watching eating videos more frequently than Australian participants, while Australian participants displayed more body image and eating concerns. Further research is needed to understand the direction of the relationships examined and how individuals from different cultural backgrounds may be influenced by social media eating videos. There is also a need for interventions that target problematic eating videos.

Evidence boundary: 自报横断面数据不能确定时间顺序或因果，样本以年轻女性为主。

Source: PMID:42508136

Observation

2026-07-28

Asia/Shanghai

Latest visible source

2026-07-27

not a 28 July publication claim

Selected

primary-source and boundary screen

Same-day batch

unverified

a failed endpoint is not a zero-result claim

METHOD / NEXT

Network toxicology and in vitro data suggest PPARA and NFE2L2 as potential key targets in TCDD-induced non-alcoholic fatty liver disease.

2,3,7,8-Tetrachlorodibenzo-p-dioxin (TCDD) is a persistent environmental pollutant linked to metabolic disorders, but its role in non-alcoholic fatty liver disease (NAFLD) remains unclear. This study integrated network toxicology, transcriptomics, and in vitro experiments to investigate the molecular mechanisms linking TCDD exposure to NAFLD. Using multi-source databases (CTD, PubChem, STITCH, SwissTargetPrediction) and two liver transcriptome datasets (GSE126848, GSE213621), we identified 176 common targets between TCDD and NAFLD, enriched in lipid metabolism, oxidative stress, inflammation, and PPAR/AhR pathways. Protein‑protein interaction network and cytoHubba algorithms prioritized ten hub genes, including PPARA, NFE2L2, IL6, and CYP1A1. Molecular docking predicted strong binding affinities of TCDD to PPARA (- 8.5 kcal/mol) and NFE2L2 (- 8.1 kcal/mol). In vitro experiments using HepG2 cells showed that TCDD dose‑dependently downregulated PPARA, NFE2L2, and their downstream targets CPT1A and NQO1, while upregulating IL6. TCDD also increased malondialdehyde (MDA) and triglyceride (TG) levels, indicating oxidative stress and lipid accumulation. These experimental results are consistent with the computational predictions. We propose a mechanistic framework in which TCDD may impair PPARA‑mediated fatty acid oxidation and NFE2L2‑mediated antioxidant defense, while promoting inflammation, thereby contributing to NAFLD. This study provides a systematic understanding of TCDD‑induced NAFLD and suggests PPARA and NFE2L2 as potential key targets for environmental risk assessment and therapeutic intervention.

Evidence boundary: 网络、对接和细胞结果构成机制假说，不能推导人群风险或治疗有效性。

Source: PMID:42508109

Consuming my body weight in videos: The relationships between watching social media eating videos, body image and eating behaviour among Australian and Chinese individuals.

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